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Recent advances in radioimmunoassay technology for the juvenile hormones

โœ Scribed by Walter G. Goodman; Anthony P. Orth; Yock C. Toong; Richard Ebersohl; Kiyoshi Hiruma; Noelle A. Granger

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
833 KB
Volume
30
Category
Article
ISSN
0739-4462

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โœฆ Synopsis

Recent refinements in juvenile hormone radioimmunoassay technology now make this method significantly more sensitive and easier to use. Rabbit polyclonal antisera against (1OR) J H Ill and racemic J H II have been developed to determine hemolymph hormone titers in the low picogram range. The antisera display minimal cross-reactivity with JH metabolites, JH analogs, and hemolymph lipids. One antiserum recognizes racemic JH I, II, and (1OR) Ill almost equivalently, exhibiting 50% displacement between 100 and 130 pg per tube. Another antiserum is JH Il-specific and exhibits 50% displacement at 35 pg per tube. Assay sensitivity has been enhanced by using (1OR,11$ [methyL3H]-JH II of very high specific activity (> 80 Ci/mmol) generated with Hyalophora cecropia accessory gland S-adenosylmethionine transferase and S-[methyl-3H]-adenosyl-L-methionine.

Preparation of biological samples has been simplified with overall recoveries of J H from hemolymph ranging between 60 and 75%.

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