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Real-Time PCR Determination of Escherichia coli Genomic DNA Contamination in Plasmid Preparations

✍ Scribed by Adrián Vilalta; Vanessa Whitlow; Terrie Martin

Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
64 KB
Volume
301
Category
Article
ISSN
0003-2697

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✍ Carl A Gregory; Gordon P Rigg; Christopher M Illidge; Ruth C Matthews 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 116 KB

This study describes the development of a novel assay for the quantification of Escherichia coli genomic DNA contamination in recombinant protein samples. The technique is based on PCR amplification and digoxygenin labeling of the genes encoding 5S ribosomal RNA followed by affinity-based collection