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REAL-TIME PCR ANALYSIS DETECTION OF AZF MICRODELETIONS: A RAPID AND ACCURATE METHOD FOR INFERTILITY SCREENING

✍ Scribed by Mielnik, Anna; Gimenez, Elena; Schlegel, Peter N; Paduch, Darius A

Book ID
122551182
Publisher
Lippincott Williams and Wilkins
Year
2009
Tongue
English
Weight
50 KB
Volume
181
Category
Article
ISSN
0022-5347

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## Abstract We describe the use of quantitative PCR (QPCR) to titer recombinant baculoviruses. Custom primers and probe were designed to gp64 and used to calculate a standard curve of QPCR derived titers from dilutions of a previously titrated baculovirus stock. Each dilution was titrated by both p