Real-Time Monitoring of Mass-Transport-Related Enzymatic Reaction Kinetics in a Nanochannel-Array Reactor

Kinetic Monitoring of Enzymatic Reaction

Kinetic Monitoring of Enzymatic Reactions in Real Time by Quantitative High-Performance Liquid Chromatography-Mass Spectrometry

✍ F.Y.L. Hsieh; X. Tong; T. Wachs; B. Ganem; J. Henion 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 428 KB

The study of enzyme kinetics under steady-state conditions represents a common and very useful method for investigating the mechanisms of enzymatic reactions. We report the use of mass spectrometry (MS) coupled with HPLC for the kinetic analysis of enzymatic reactions in real time. The hydrolysis of