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Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer

โœ Scribed by Dogra, Navneet (author);Reyes, Julia C. (author);Garg, Nishi (author);Kohli, Punit (author)


Book ID
120344641
Publisher
MyJoVE Corporation
Year
2012
Tongue
English
Weight
536 KB
Volume
66
Category
Article
ISSN
1940-087X

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When capture oligonucleotides are tethered on planar surfaces, mass transport limitations influence the kinetics of solid-phase nucleic acid hybridizations. By diffusion theory, however, hybridization of oligonucleotides on microparticles should be reaction-rate limited. In an initial effort to unde