โ Scribed by Steven C. Hill; Ronald G. Pinnick; Stanley Niles; Yong-Le Pan; Stephen Holler; Richard K. Chang; Jerold Bottiger; Bean T. Chen; Chun-Sing Orr; Greg Feather
No coin nor oath required. For personal study only.
Improved real-time methods for characterizing airborne biological particles are needed. Here we review our efforts in developing techniques for measuring the laser-induced fluorescence (total and spectrally dispersed) of individual airborne particles, and describe our present system, which can measure fluorescence spectra of single micrometer-sized bioaerosol particles with good signal-to-noise ratios. We demonstrate the capability of this system by showing measured spectra of a variety of airborne particles generated in the laboratory from road dust, ammonium sulfate, Bacillus subtilis and other bacteria prepared under various conditions, allergens, cigarette smoke, and chicken-house dust. These spectra illustrate the capability of the system to distinguish between some biological and nonbiological aerosols, and among several types of laboratory-generated biological aerosols. We suggest improvements needed to make our system field portable.
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Background and Objective: This in vivo study examines the validity of using fluorescence measurements of laser-induced release of temperature-sensitive, liposome-encapsulated dye for real-time monitoring of temperature and for prediction of tissue thermal damage. ## Study DesignlMaterials and Metho