Real-time measurement of cytosolic free calcium concentration in HL-60 cells during static magnetic field exposure and activation by ATP

✍ Scribed by Michelle Belton; Kristy Commerford; Justin Hall; Frank S. Prato; Jeffrey J.L. Carson

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 155 KB
• Volume: 29
• Category: Article
• ISSN: 0197-8462
• DOI: 10.1002/bem.20409

✦ Synopsis

Calcium ions are involved in a number of important signal transduction pathways in cells. Cytosolic calcium concentration ([Ca 2þ ] c ) can be affected by the activation of Ca 2þ channels through the action of ligands such as ATP. The response of [Ca 2þ ] c to ligands may be affected by external factors like magnetic fields. The purpose of this study was to determine if exposure to a static magnetic field (SMF) for 800 s altered the [Ca 2þ ] c response to ATP in undifferentiated HL-60 cells. We sham exposed or field exposed fura-2 loaded HL-60 cells to a SMF of 1, 10, and 100 mT. Cells were activated with ATP 300 s into the exposure. The level of [Ca 2þ ] c was followed before, during, and after field or sham exposure with a ratiometric fluorescence spectroscopy system. It was found that high concentrations of ATP resulted in greater [Ca 2þ ] c responses, but faster recovery to near basal levels. The application of 1, 10, or 100 mT SMF did not affect the [Ca 2þ ] c response to ATP. Future work could examine the effect of a longer SMF exposure on the [Ca 2þ ] c response to ATP. Longer exposures might provide sufficient time for morphological changes in the plasma membrane to occur.