Real time analysis of diffraction patterns, at extremely low light levels

Routes to improving the reliability of l

Routes to improving the reliability of low level DNA analysis using real-time PCR

✍ Stephen LR Ellison; Claire A English; Malcolm J Burns; Jacquie T Keer 📂 Article 📅 2006 🏛 BioMed Central 🌐 English ⚖ 762 KB

## Background Accurate quantification of DNA using quantitative real-time PCR at low levels is increasingly important for clinical, environmental and forensic applications. At low concentration levels (here referring to under 100 target copies) DNA quantification is sensitive to losses during prepa