𝔖 Bobbio Scriptorium
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Reagentless fluorescent biosensors based on proteins for continuous monitoring systems

✍ Scribed by Javier Galbán; Isabel Sanz-Vicente; Estefania Ortega; Melisa del Barrio; Susana de Marcos


Book ID
113021858
Publisher
Springer
Year
2012
Tongue
English
Weight
540 KB
Volume
402
Category
Article
ISSN
1618-2650

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The galactose/glucose-binding protein (GBP) is synthesized in the cytoplasm of Escherichia coli in a precursor form and exported into the periplasmic space upon cleavage of a 23-amino-acid leader sequence. GBP binds galactose and glucose in a highly specific manner. The ligand induces a hinge motion