Reactivity of synthetic peptides representing selected sections of hepatitis C virus core and envelope proteins with a panel of hepatitis C virus-seropositive human plasma
✍ Scribed by Jackson, Peter; Petrik, Juraj; Alexander, Graeme J. M.; Pearson, Gavin; Allain, Jean-Pierre
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 160 KB
- Volume
- 51
- Category
- Article
- ISSN
- 0146-6615
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✦ Synopsis
A series of 54 synthetic peptides, 15-20 residues ease is usually asymptomatic in the early stages but often progresses to a chronic infection and can result long, that represented selected parts of the structural proteins of hepatitis C virus (HCV) were in hepatocellular carcinoma [Hopf et al., 1990; Choo et al., 1991; Esteban et al., 1991]. The viral genome is a tested for immunoreactivity with a panel of 45 plasma samples from potential blood donors single-stranded positive polarity RNA virus 9.5 kb long that codes for a polyprotein of approximately 3,000 who were known to be seropositive for anti-HCV. Most of the ten peptides that represented the amino acid residues that includes three structural proteins, core and envelope glycoproteins E1 and E2, and core protein showed reactivity with most of the panel samples. All except one of the 20 peptides at least nine non-structural proteins that mediate replication [Choo et al., 1991;. It has six that represented non-hypervariable regions of envelope proteins E1 and E2 showed little or no major genotypes and at least 11 subtypes . In addition, the E2 pro-reactivity. In contrast, 18 of the the 24 peptides that represented variants of the hypervariable tein has two short lengths of high sequence variability, hypervariable regions (HVR) 1 and 2 [Hijikata et al., region 1 of the E2 protein reacted with at least one panel sample. Notably, 40% of the panel 1991; Kato et al., 1991]. Isolates of HCV show markedly different sequences in these regions, and single samples samples cross-reacted with two or more different peptides sequences some of which differed by often contain multiple closely related variants or quasispecies which may alter in a single patient during the more than 50%. Two panel samples each crossreacted with seven different peptide sequences.