Reactivities of Organic Phase Biosensors 3: Electrochemical Study of Cytochrome P450cam Immobilized in a Methyltriethoxysilane Sol-Gel
✍ Scribed by Emmanuel I. Iwuoha; Stephen Kane; Conchita Ovin Ania; Malcolm R. Smyth; Paul R. Ortiz de Montellano; Uwe Fuhr
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 340 KB
- Volume
- 12
- Category
- Article
- ISSN
- 1040-0397
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✦ Synopsis
A composite amperometric biosensor was prepared by encapsulating a cytochrome P450 cam adidodecyldimethylammonium bromide (DDAB) liquid crystal system in a methyltriethoxysilane (MTEOS) sol-gel (SG). The composite sensing layer was cross-linked unto a 0.071 cm 2 glassy carbon electrode (GCE) using glutaraldehyde. The electrochemical characteristics of the GCE-[P450 cam aDDAB/SG] biosensor were studied by multiscan cyclic voltammetry in aqueous phase (0.05 phosphate buffer pH 7.3) and organic (CH 3 CN : H 2 0; 90 % : 10 %) phase anaerobic media. Analysis of the cyclic voltammetric results show that the sensor exhibited faster reversible electrochemistry in the organic phase than in the aqueous phase. The cyclic voltammetric peak separation, DE p , values estimated for the buffer and acetonitrile media were 62 mV and 104 mV, respectively. The sol-gel-based sensor had a life-time of 4 weeks when stored at 4 C, and was stable up to 45 C, which is also the temperature of optimal sensor activity.