Reactive oxygen species inducible by low-intensity laser irradiation alter DNA synthesis in the haemopoietic cell line U937
✍ Scribed by Callaghan, Gary A.; Riordan, Cathal; Gilmore, William S.; McIntyre, Irene A.; Allen, James M.; Hannigan, Bernadette M.
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 694 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0196-8092
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✦ Synopsis
Background and Objective: The purpose of this study was to evaluate the possible role of reactive oxygen species (ROS) in mediating previously recorded alterations in DNA synthesis, inducible by low-intensity laser irradiation (LILI), in the haemopoietic cell line U937. Study DesigdMateriaZs and Methodp: The ability of LILI (660 nm, 12 mW, 5 kHz) to induce ROS from U937 cells was assessed spectrophotometrically at energy densities (E.D.) from 1.0 to 11.5 J/cm2. In order to assess whether laser-induced ROS could alter cellular proliferation DNA synthesis was measured post-irradithe cells in both the presence and absence of the antioxidant catalase (CAT). Results: Detectable ROS were produced post-irradiation only from the differentiated form of the cell line. Analysis by Student's t-test for unrelated groups showed a significant difference, at E.D.s 2.9 and 8.6 J/cm2, in the extent of DNA synthesis occurring in cells irradiated in the presence of CAT or in its absence. Conclusion: These findings demonstrate that laser-inducible ROS can mediate laser's effects on this cell line.