Reactive groups on polymer covered electrodes, 4. Lactate-oxidase-biosensor based on electrodes modified by polythiophene

✍ Scribed by Hans-Peter Welzel; Gerhard Kossmehl; Gunnar Engelmann; Barbara Neumann; Ulla Wollenberger; Frieder Scheller; Werner Schröder

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 675 KB
• Volume: 197
• Category: Article
• ISSN: 1022-1352
• DOI: 10.1002/macp.1996.021971023

✦ Synopsis

Abstract

Electrocopolymerization of 3‐thiopheneacetic acid (1) and 3‐methylthiophene (2) under various conditions produces poly{(3‐methylthiophene‐2,5‐diyl)‐co‐[3‐(carboxymethyl)thiophene‐2,5‐diyl]} (3). By activation of the carboxy groups with dicyclohexylcarbodiimide (DCC) lactate oxidase (LOD) is covalently bonded to the surface of the electrode. In this way a lactate sensor was formed which is applicable for the determination of lactate in micromolar concentrations.