N-benzoyl-L-phenylalanyl-L-phenylalanine is an excellent peptide substrate for carboxypeptidase A; at 30ยฐC and pH 7.5, K m is 2.6 ร 10 -5 M while k cat is 177 s -1 (k cat /K m = 6.8 ร 10 6 M -1 s -1 ). Indole-3-acetic acid is a noncompetitive or mixed inhibitor towards the peptide and toward hippury
โฆ LIBER โฆ
Reaction of peptide thiobenzyl esters with mammalian chymotrypsinlike enzymes: A sensitive assay method
โ Scribed by J.Wade Harper; Guillermo Ramirez; James C. Powers
- Book ID
- 102626568
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 404 KB
- Volume
- 118
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
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The orientation of an immobilized antigen is important for recognition by, e.g., an antibody. When noncovalent passive adsorption is used for immobilization, the number of ways that the antigen can attach to the surface is numerous and control of how the antigen orientates on the surface is limited.