Rational design of13C-labeling experiments for metabolic flux analysis in mammalian cells
β Scribed by Scott B Crown, Woo Suk Ahn, Maciek R Antoniewicz
- Book ID
- 119912025
- Publisher
- BioMed Central
- Year
- 2012
- Tongue
- English
- Weight
- 391 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1752-0509
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract This contribution addresses the identification of metabolic fluxes and metabolite concentrations in mammalian cells from transient ^13^Cβlabeling experiments. Whilst part I describes experimental setβup and acquisition of required metabolite and ^13^Cβlabeling data, part II focuses on s
## Abstract Metabolic flux analysis (MFA) is widely used to quantify metabolic pathway activity. Typical applications involve isotopically labeled substrates, which require both metabolic and isotopic steady states for simplified data analysis. For bacterial systems, these steady states are readily