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Rational and systematic protein purification process development: the next generation

✍ Scribed by Beckley K. Nfor; Peter D.E.M. Verhaert; Luuk A.M. van der Wielen; Jürgen Hubbuch; Marcel Ottens


Book ID
113932271
Publisher
Elsevier Science
Year
2009
Tongue
English
Weight
284 KB
Volume
27
Category
Article
ISSN
0167-7799

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## Abstract In this paper, a wide range of antibodies from various subclasses and subfamilies are employed to evaluate the creation of generic separation processes using Protein A chromatography. The reasons for elution pH differences amongst several IgG1s, IgG2s, antibody fragments, and Fc‐fusion