Rates of exchange of phosphate ions in human, rabbit, and chicken blood

✍ Scribed by Gourley, D. R. H. ;Matschiner, J. T.

• Publisher: Wiley (John Wiley & Sons)
• Year: 1953
• Tongue: English
• Weight: 567 KB
• Volume: 41
• Category: Article
• ISSN: 0095-9898
• DOI: 10.1002/jcp.1030410204

✦ Synopsis

T W O FIGUKER

Considerable success has been achieved in quantitative studies of the exchange of cations between the plasma and erythrocytes of various species iia vitro (Sheppard and Martin, '50 ; Sheppard, nlartin and Beyl, '51 ; Raker, Taylor, Weller and Hastings, '50). Although the kinetics involved in cation exchange are by no means simple, a study of the exchange of orthophosphate anions in such systems presents additional difficulties. For example, exchange of potassium ions between plasma and blood cells lends itself very well to kinetic analysis based upon a two-compartment system (Sheppard and Martin, '30). However, when phosphatc ions enter blood cells, they quickly become bound wit11 organic moieties to form phosphate esters which occur only intracellularly. Thus in addition to two major compartments, the cells and the plasma, there may be in the case of phosphate ions an unknown number of minor "compartni~nts" consisting of the various phosphate esters in the cells.

Kinetic analysis of the exchange of potassium ions between blood cells and plasma is further simplified by the fact that it is possible to maintain this system in v i t r o in a biologically steady state, as shown by relatively constant cell and plasma potassium contents f o r several hours. I n the case of phosphate ions This work was carried out under contract No. AT-(40-1)-263 with the Medical Branch, Division of Biology and Medicine of the U. S. Atomic Energy Commission. . I preliminary report has been published (Federation Proc., I 2 : 57, 1952).