Rate of RNA synthesis and tRNA end-labeling during early development ofPhaseolus

✍ Scribed by Virginia Walbot

• Book ID: 104749801
• Publisher: Springer-Verlag
• Year: 1972
• Tongue: English
• Weight: 596 KB
• Volume: 108
• Category: Article
• ISSN: 0032-0935
• DOI: 10.1007/bf00386078

✦ Synopsis

Axes of Phaseolus vulgaris cease synthesis of RNA during the maturation stage of embryogeny. During the imbibition phase of germination RNA synthesis resumes after the axes reach a normal water content. In the first hour of imbibition a very low rate of incorporation of all-adenosine into is RNA found, and the primary site of incorporation is the -CCA end of tRNA. At later stages of germination tRNA end-labeling accounts for a minor fraction of adenosine incorporation. The rate of RNA synthesis increases after initiation of axis elongation a maximal rate at 18 h of germination. ATP pool-size and specific activity vary over a several-fold range during development, an important consideration in determining the rate of RNA synthesis.