Rat monoclonal antibodies to the external domain of the product of the C-erbB-2 proto-oncogene

✍ Scribed by Jennifer M. Styles; Shona Harrison; B. A. Gusterson; C. J. Dean

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: French
• Weight: 655 KB
• Volume: 45
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910450219

✦ Synopsis

With the breast carcinoma cell line BT 474 used as a source of antigen, four rat monoclonal antibodies (MAbs) (3 IgG,, and I IgA) have been prepared against the external domain of the product of the c-erbB-2 proto-oncogene. All 4 antibodies stain frozen sections of tissues that over-express the product of the c-erb8-2 proto-oncogene, and competitive binding as- says showed that the antibodies recognized 2 non-overlapping epitopes. Representative antibodies from the two groups (ICRI2 and 13) were shown to specifically immunoprecipitate a IPOkDa protein from 3SS-methionine-labelled breast carcinoma cells where the c-erbB-2 is amplified (BT 474 and MDA-MB 361). Two of the antibodies (ICRIZ and ICR17) bind to the denatured antigen in Western blots and lCRl2 stains formolsaline-fixed sections of breast carcinoma tissue that overexpresses the product of the c-erbB-2 proto-oncogene. These antibodies should be useful not only for immunocytochemical diagnoses but also for radio-irnmunoscintigraphic and therapeutic applications.