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Rat hepatocyte plasma membrane acyl:CoA synthetase activity

✍ Scribed by B. C. Davidson; R. C. Cantrill


Book ID
112772416
Publisher
Springer-Verlag
Year
1986
Tongue
English
Weight
388 KB
Volume
21
Category
Article
ISSN
0024-4201

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A fluorimetric assay for acyl-CoA synthe
✍ Wessel Lageweg; Ivar Steen; Joseph M. Tager; Ronald J.A. Wanders πŸ“‚ Article πŸ“… 1991 πŸ› Elsevier Science 🌐 English βš– 415 KB

In this paper we describe a fluorimetric assay for the measurement of long-chain acyl-CoA synthetase activity in rat liver postnuclear supernatants. The method is based upon the use of acyl-CoA oxidase which catalyzes the dehydrogenation of acyl-CoA esters to yield enoyl-CoA esters and H2O2. H2O2 su