Rat and mouse identifier sequences are preferentially but not exclusively located in cortical neuronal genes expressed postnatally
✍ Scribed by I. R. Brown; P. Danielson; S. Rush; M. Godbout; J. G. Sutcliffe
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 650 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0360-4012
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✦ Synopsis
A genetic element called the identifier (ID) sequence, highly repeated in the rat genome, has previously been reported to be located in the introns of some genes transcribed in the adult rat brain by RNA polymerase I1 (Pol 11). We show that nuclear RNA isolated from neurons of cerebral hemispheres (cortex) of 14day old rats is enriched more than 10-fold in ID sequences compared to nuclear RNA from liver, kidney, cerebellum, or cortical glia. The developmental onset of the difference is during the first 2 weeks after birth. Mouse cortical neuronal nuclear RNA is similarly enriched in an element related but not identical to the rat ID element, and the enrichment also has postnatal onset. The enriched appearance of ID sequences in transcripts whose expression is increased postnatally in cortical neurons correlates developmentally and spatially with the transcription of ID elements by RNA polymerase 111 (Pol IIT) and with a change in chromatin structure.