✦ LIBER ✦
RASAcontains a polymorphic microsatellite and maps to bovine syntenic group U22 on Chromosome 7q2.4-qter
✍ Scribed by André Eggen; Sabina Solinas-Toldo; Allan B. Dietz; James E. Womack; Gerald Stranzinger; Ruedi Fries
- Publisher
- Springer-Verlag
- Year
- 1992
- Tongue
- English
- Weight
- 772 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0938-8990
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✦ Synopsis
The bovine gene for the p21ras protein activator (RASA) includes in its 5' untranslated region a (TG)n repeat. Analysis of this (TG)n repeat by PCR amplification of genomic DNA revealed a four-allele polymorphism. A cDNA probe was used to assign RASA to the region 2.4-qter of bovine Chromosome (Chr) 7 by in situ hybridization. PCR analysis of a panel of somatic hybrid lines allowed the assignment of RASA to the unassigned syntenic group 22 (U22) and thus localizes U22 on Chr 7.