RAS gene activation in acute myelogenous leukemia: Analysis by in vitro amplification and dna base sequence determination
✍ Scribed by Shrikant M. Mane; Stephen J. Meltzer; John C. Gutheil; Vikram Kapil; Edward J. Lee; Dr. Samuel W. Needleman
- Book ID
- 102844483
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 641 KB
- Volume
- 2
- Category
- Article
- ISSN
- 1045-2257
No coin nor oath required. For personal study only.
✦ Synopsis
RAS protooncogene activation has been repeatedly demonstrated in neoplastic cell
D N A from patients with AML. Despite the convincing demonstration that activating RAS gene point mutations are critical in model systems, their precise prevalence and importance in human cancers such as AML remain speculative. The technology for identifying RAS mutations has changed considerably in recent years. We examined a prospective cohort of 43 acute myeloid leukemia (AML) patients admitted to the University of Maryland Cancer Center for first and second exon mutations of NRAS and KRAS using PCR and D N A sequence analysis. Six (I 4%) I st exon NRAS mutations were identified. N o clinical or biologic parameter has yet been observed to segregate with RAS activation, although a larger study may be needed to demonstrate this.