Rare alleles at different VNTR loci among lung-cancer patients with microsatellite instability in tumours
✍ Scribed by Bjørn-Arne Lindstedt; David Ryberg; Aage Haugen
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- French
- Weight
- 54 KB
- Volume
- 70
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
Work in our laboratory has shown a significantly higher frequency of microsatellite mutations in tumours from lungcancer patients with rare alleles at the Hras1 VNTR locus compared with those with common alleles. In 137 lungcancer patients, the association between microsatellite instability and rare alleles at the Hras1 VNTR locus was confirmed with 17 microsatellite markers. We found a significant association between LOH in lung tumours of marker D3s966 with microsatellite instability. In samples with LOH at marker D3s966 (3p21.3) 22% of loci tested showed instability, whereas 8% showed instability without LOH at D3s966. To investigate whether rare alleles at the Hras1 locus are linked to rare alleles at other loci, a second minisatellite (D17S4) was genotyped. In a population of 406, 4 individuals with D17S4 rare alleles were detected of whom 3 also had rare alleles at the Hras1 VNTR locus. The probability of this association to occur by chance is low. Thus, rare alleles at the Hras1 locus may be associated with rare alleles at other loci, and could be an indication of germline instability. The findings indicate that microsatellite instability in lung tumours is not strictly associated with features in the Hras1 proto-oncogene, but may be the result of the same mechanism(s) that generate(s) new alleles at the Hras1 and D17S4 loci.