Rapidly dissolving forms of digoxin: Hydroquinone complex
✍ Scribed by Takeru Higuchi; Masaru Ikeda
- Book ID
- 102402711
- Publisher
- John Wiley and Sons
- Year
- 1974
- Tongue
- English
- Weight
- 327 KB
- Volume
- 63
- Category
- Article
- ISSN
- 0022-3549
No coin nor oath required. For personal study only.
✦ Synopsis
be free of endotoxin, e.g., by treating this carefully cleaned equipment at 180" for several hours.) Ten microliters of the sample is added and mixed with the aid of the capillary tube (a special pipet holder with a punched rubber cap is recommended3). A negative control and a positive control are placed in the same manner on two separate test fields. Air bubbles should be prevented or eliminated using a needle, which has been previously heated to red heat and then allowed to cool. All of the small droplets placed on a slide must show some slight movement when the plate is gently vibrated. The slide is then placed in a "moist chamber" consisting, for example, of a flat plastic box, the bottom of which has been covered with a piece of moistened cellulose. Two glass rods serve as support of the slide.
The whole assembly is incubated at 37" for 30 min. After 30 min, the slide is observed against a bright background. "he slide is always held in a horizontal position and may be gently tilted or vibrated through an angle of a few degrees only. A solid gel or an increase in viscosity is easily detected. The incubation may be prolonged, but usually the result remains unchanged. Very hygroscopic solutions tend to increase the volume of the droplets upon prolonged standing and, therefore, to reduce the sensitivity of the test. A nonsaturated moist chamber might be used in these cases.