✦ LIBER ✦

Rapid typing of Bacillus subtilis strains by their secondary metabolites using matrix-assisted laser desorption/ionization mass spectrometry of intact cells

✍ Scribed by Frank Leenders; Torsten H. Stein; Bärbel Kablitz; Peter Franke; Joachim Vater

Book ID: 101238020
Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 105 KB
Volume: 13
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(19990530)13:10<943::aid-rcm591>3.0.co;2-0

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✦ Synopsis

Matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry (TOFMS) has been used for rapid and efficient typing of Bacillus subtilis strains according to their secondary metabolite products using whole intact cells as targets. Cells were grown either in liquid media or on agar plates, respectively. Strains were classified by their unique spectra of bioactive peptides in the m/z range from 500 to 5000. The lipopeptide products surfactin, fengycin and the members of the iturin family were structurally characterized in situ by post-source decay (PSD) analysis. The potential of MALDI-MS for taxonomic studies, as well as for the investigation of the regulation and compartimentalization of secondary metabolites in the bacterial cell, is demonstrated. These achievements qualify this methodology for rapid screening of microorganisms to exploit bioactive compounds for biotechnological and pharmaceutical applications.

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