Rapid Synthesis of a DNA Competitor for Use in Quantitative Polymerase Chain Reaction Analysis Using Simple Molecular Techniques

✍ Scribed by Luke van Leeuwen; Ann K. Guiffre; William A. Sewell; Kerry Atkinson

• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 80 KB
• Volume: 243
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1996.0505

✦ Synopsis

10). This indicated that nonspecific degradation was DNA in the lower part of the gel. As a general conclusion, in order to not obscure similar analyses by the not the case for the observed anomalous appearance of our samples. We could also exclude agarose quality, phenomenon we described here, we recommend staining of the gel only after run to visualize larger amounts electrophoresis conditions, the particular restriction enzyme, and the source of DNA as possible causes for of DNA. the phenomenon (data not shown). We next examined Acknowledgments. This work was supported by a grant of the whether the anomalous appearance of the genomic Deutsche Forschungsgemeinschaft to H.Z. (Zo 59/2-2). We thank Monika Niggemann for technical help and Prof. Dr. E.-L. Winnacker DNA was due to the presence of ethidium bromide durfor continuous support.

ing the run. To this purpose, we performed an electrophoretic analysis of genomic DNA from CHO cells and REFERENCES from chicken erythrocytes, cleaved with AluI or MboII, in either the presence or absence of ethidium bromide 1.