Rapid sulfopropyl-disk chromatographic purification of bovine and human thrombin

✍ Scribed by Frank C. Church; Herbert C. Whinna

• Book ID: 102986198
• Publisher: Elsevier Science
• Year: 1986
• Tongue: English
• Weight: 681 KB
• Volume: 157
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(86)90198-3

✦ Synopsis

Thrombin, from either a crude commercial preparation (bovine) or a prothrombin activation mixture (human), was purified by sulfopropyldisk chromatography to homogeneity in a rapid and convenient single-step procedure. The yield of both proteinases was greater than 85%. Purified bovine and human thrombin had sp act of 2500 and 3000 "NIH" units/m& respectively. Human thrombin was more reactive than bovine thrombin with these active site-directed probes: phenylmethylsulfonyl tluoride, dansyl-GluGly-Arg-chloromethylketone, and human heparin cofactor II. The sulfopropyl-disk chromatographic method is a useful and rapid technique to prepare milligram quantities of highly purified bovine and human thrombin. o 1986 Academic PKXS, I~C.