Rapid Solid-phase Extraction/Derivatization System for Sample Preparation and Gas Chromatographic/Mass Spectrometric Determination of Drugs in Human Urine

✍ Scribed by Soledad Cárdenas; Mercedes Gallego; Miguel Valcárcel

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 216 KB
• Volume: 11
• Category: Article
• ISSN: 0951-4198
• DOI: 10.1002/(sici)1097-0231(19970215)11:3<298::aid-rcm775>3.0.co;2-#

✦ Synopsis

A novel automatic system has been developed for the rapid solid-extraction and derivatization of different drugs and their metabolites from urine, prior to further analysis. Manual injection of underivatized/derivatized drugs in a GC/MS system, in which the quadrupole mass filter was operated conventionally, provided high sensitivity and selectivity at the selected m/z value. The precision (2.1-6.8%) is acceptable and the overall recovery from urine was determined to be ca. 95%. The effectiveness of this approach is demonstrated with data obtained from different urine samples which had tested positively.