In order to perform the immune complex transfer enzyme immunoassays for HIV-1 p24 antigen and antibody IgGs to HIV-1 p17, reverse transcriptase and gp41 antigens as rapidly as possible, methods for rapid formation of the immune complexes on solid phase are described. HIV-1 p24 antigen was reacted wi
โฆ LIBER โฆ
Rapid solid-phase enzyme immunoassay for antibodies to viruses and other microbes: effects of polyethylene glycol
โ Scribed by Eeva-Marjatta Salonen; Antti Vaheri
- Book ID
- 118898399
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 462 KB
- Volume
- 41
- Category
- Article
- ISSN
- 0022-1759
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In a previously reported ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) for antibody IgG to HIV-1, polystyrene beads in test tubes were handled with tweezers, and bound P-D-galactosidase activity was measured with a fluorometer. The use of tweezers was causative of fa