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Rapid small scale preparation of bacterial genomic DNA, suitable for cloning and hybridization analysis

โœ Scribed by J. LEWINGTON; S. D. GREENAWAY; B. J. SPILLANE


Book ID
115271313
Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
316 KB
Volume
5
Category
Article
ISSN
0266-8254

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## Background Current methods for accurate quantification of nucleic acids typically begin with a template preparation step in which DNA and/or RNA are freed of bound proteins and are then purified. Isolation of RNA is particularly challenging because this molecule is sensitive to elevated temperat