Rapid, simple and sensitive microassay for skeletal muscle homogenates in the functional assessment of the Ca-release channel of sarcoplasmic reticulum: Application to diagnosis of susceptibility to malignant hyperthermia

Microassay for malignant hyperthermia su

Microassay for malignant hyperthermia susceptibility: hypersensitive ligand-gating of the Ca channel in muscle sarcoplasmic reticulum causes increased amounts and rates of Ca-release

✍ Peter J. O'Brien 📂 Article 📅 1990 🏛 Springer 🌐 English ⚖ 490 KB

A crude preparation of heavy sarcoplasmic reticulum (HSR) was isolated using 1 gram of muscle obtained from swine susceptible to malignant hyperthermia (MH) and from control swine. The caffeine and ATP concentration-dependency of Ca-release was determined using suction filtration with radioisotopic