Rapid separation of peptides and proteins on 2-μm porous microspherical reversed-phase silica material
✍ Scribed by Hiroyuki Moriyama; Masakazu Anegayama; Yoshio Kato
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 303 KB
- Volume
- 729
- Category
- Article
- ISSN
- 1873-3778
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Octadecyl-modified 1.5 µm diameter non-porous silica particles were packed in 150 µm i.d. (360 µm o.d.) capillaries with lengths of 20 cm which were used to separate proteins and peptides generated from enzymatic digests of proteins. Gradients were produced using an exponential dilution method at pr
Following previous studies of the use of non-porous monodisperse 1.5-pm noctyl-and n-octadecyl-bonded silicas in gradient elution of proteins, this work was aimed at elucidating further the properties of this novel column material for peptide and protein separations in comparison with wide-pore sili