✦ LIBER ✦

Rapid separation of mouse T and B lymphocytes using wheat germ agglutinin

✍ Scribed by Lilly Y. W. Bourguignon; Richard L. Rader; James T. McMahon

Publisher: John Wiley and Sons
Year: 1979
Tongue: English
Weight: 423 KB
Volume: 99
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040990111

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✦ Synopsis

Abstract

A separation procedure has been developed for mouse splenic T and B lymphocytes which is based on their differential agglutination by wheat germ agglutinin (WGA). In the presence of 50‐100 μg/ml of WGA, multicellular aggregates are formed which are enriched in B cells. These aggregates can be separated from monodisperse T cells by gravity sedimentation and subsequently dissociated into single cells by treatment with N‐acetylglucosamine (NAG). Immunocytochemical analyses and mitogenic assays indicate approximately 10‐15% cross contamination of the resultant B and T cell fractions. The separation procedure is not only convenient and rapid but also allows the simultaneous recovery of viable T and B cells from the same spleen preparation.

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