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RAPID SEPARATION AND DETECTION OF CONCANAVALIN A REACTING GLYCOPROTEINS: APPLICATION TO STORAGE PROTEINS OF A LEGUME SEED

✍ Scribed by MARCELLO DURANTI; SHELA GORINSTEIN; PAOLO CERLETTI


Book ID
111336891
Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
368 KB
Volume
14
Category
Article
ISSN
0145-8884

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Glycoproteins in nitrocellulose transfers of electrophoretically separated mixtures of cellular and viral proteins are rapidly and sensitively located by sequential incubation with the lectin concanavalin A and the enzymatically active glycoprotein horseradish peroxidase. The bound enzyme is located