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Rapid, sensitive detection of plasma IL-1 by extraction and bioassay

✍ Scribed by J.J. Series; D. Shapiro; I. Cameron; J. Smith; W.D. Fraser; O.J. Garden; A. Shenkin


Book ID
118901308
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
300 KB
Volume
108
Category
Article
ISSN
0022-1759

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A method is described which permits the assay of 1,25dihydroxyvitamin D in 1 ml of normal human plasma. Samples are extracted with benzene or isopropyl ether. The organic phase is then back-extracted with 0.1 M NhHPO,, pH 10.5. The final purification step involves high-pressure liquid chromatography