Rapid sedimentation of Escherichia coli in the presence of polyethylene glycol at 1g

✍ Scribed by Donald L. Eshenbaugh; Donald Sens; Eric James

• Book ID: 102625600
• Publisher: Elsevier Science
• Year: 1974
• Tongue: English
• Weight: 293 KB
• Volume: 58
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(74)90207-3

✦ Synopsis

In order to harvest a NO-liter culture of bacteria with a conventional refrigerated centrifuge, using a 1.5 or 3.0-liter capacity rotor, about 10 hr of arduous work are involved. In contrast, the procedure described using PEG results in almost quantitative recovery of viable cells, with the expenditure of less than 10% of the labor. This technique may be applied to any desired volume of culture using simple equipment which may be built in any laboratory. It should be applicable for harvesting E. co2i destined for the isolation of any stable protein, nucleic acid, or other component, including bacteriophage which carry a late lysis mutation thus preventing lysis of bacterial cells; however, this procedure may not be suitable for proteins which are not stable at 4°C for 12 hr.