Rapid removal of N-linked oligosaccharides using microwave assisted enzyme catalyzed deglycosylation

Enzyme-Catalyzed Synthesis of a Hybrid N

Enzyme-Catalyzed Synthesis of a Hybrid N-Linked Oligosaccharide using N-Acetylglucosaminyltransferase I

✍ Rui Chen; Mark A. Pawlicki; Brian S. Hamilton; Thomas J. Tolbert 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 224 KB

## Abstract The soluble catalytic domain of human N‐acetylglucosaminyltransferase I was purified from __Escherichia coli__ and utilized in the enzyme‐catalyzed conversion of high mannose N‐linked oligosaccharide **1** into the rare hybrid oligosaccharide **2**. Analysis of the reaction showed that