Rapid recovery in mice after combined nasal/oral immunization with killed respiratory syncytial virus
✍ Scribed by Peter D. Reuman; Scott P. Keely; Gilbert M. Schiff
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 634 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0146-6615
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✦ Synopsis
Abstract
Based on the concept of a common mucosal immune system, the murine gastrointestinal tract was inoculated (oral) with three doses (5, 20, and 40 μg) of UV‐inactivated respiratory syncytial virus (RSV) in order to elicit a virus‐specific immune response in the respiratory tract. Only the 40 μg dose induced significant (P < 0.01) anti‐RSV‐IgG rises in serum and lung wash compared to controls. To improve the immune response, mice were immunized intranasally under light anesthesia with the same 40 μg dosage regimen of killed RSV so that each dose passed through the nose and was swallowed. This combined nasal/oral immunization stimulated anti‐RSV‐IgG in serum, lung wash and nasal wash (P < 0.001) and anti‐RSV‐IgA in lung and nasal wash (P < 0.001) that were comparable to levels after infection with live RSV. Three days after challenge with live RSV, mice given combined nasal/oral immunization showed suppressed nasal virus shedding (P = 0.025). Nasal virus shedding correlated inversely with concentrations of anti‐RSV‐Ig in nasal secretions but did not correlate with concentrations in serum.