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Rapid quantitative determination of epirubicin and its metabolites in plasma using high performance liquid chromatography and fluorescence detection

✍ Scribed by T. Dine; C. Brunet; M. Luyckx; M. Cazin; P. Gosselin; J. L. Cazin

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
364 KB
Volume
4
Category
Article
ISSN
0269-3879

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✦ Synopsis

A rapid sensitive and selective isocratic technique was developed for the analysis of plasma epirubicin and three of its known fluorescent metabolites epirubicinol, 4'-O-~-~-glucuronyl-4'-epidoxorubicin and 4'-O-fi-D-ghCUrOnyl 1,3-dihydro-4'-epidoxorubicin, with daunorubicin as an internal standard, by using high performance liquid chromatography (HPLC) with fluorescence detection and a 'Hypersil ODs' column. The drugs were easily and efficiently extracted with a Sep-Pak C,* cartridge and the mean recoveries were greater than 85%. Intraassay and Interassay coefficients of variation (plasma samples) were better than 8.25%. An example of pharmacokinetic study obtained in a cancer patient after intravenous injection of epirubicine is described.

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