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Rapid purification method of lysostaphin for analysis of cell-wall proteins

✍ Scribed by Motoyuki Sugai; Tomoko Akiyama; Yoichiro Miyake; Eisaku Ishida; Hidekazu Suginaka

Book ID
119126114
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
303 KB
Volume
12
Category
Article
ISSN
0167-7012

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Two proteins were purified from Listeria monocytogenes cell wall using detergent extraction, Superose 6 gel chromatography. Mono Q cation-exchange chromatography and Superose 12 gel chromatography. Proteins were shown to form complexes of ca. 300kDa and pI 4.7. These complexes could not be dissociat