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Rapid orientated cloning in a shuttle vector allowing modulated gene expression in Bacillus subtilis

✍ Scribed by Pascale Joseph; Jean-Raphael Fantino; Marie-Laure Herbaud; François Denizot

Book ID
117517146
Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
368 KB
Volume
205
Category
Article
ISSN
0378-1097

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✍ Kawamura, Fujio ;Saito, Hiuga ;Ikeda, Yonosuke 📂 Article 📅 1980 🏛 Springer 🌐 English ⚖ 731 KB

We attempted to use Bacillus subtilis phage phi 1 as a gene-cloning vector since the phi 1 genome was found to have few cleavage sites upon digestion with several kinds of restriction endonucleases. A phi 1 stock supplied by J. Ito (University of Arizona, Tucson, USA) consisted of two phages, phi 1E