๐”– Bobbio Scriptorium
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Rapid multiplication ofSapium sebiferumRoxb. by tissue culture

โœ Scribed by M. Kotwal Mridula; P. K. Gupta; A. F. Mascarenhas

Publisher
Springer
Year
1983
Tongue
English
Weight
977 KB
Volume
2
Category
Article
ISSN
0167-6857

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โœฆ Synopsis

Multiple shoots formation and elongation was induced from stem explants of Sapium seedlings on media containing cytokinins.

Leaf expiants produced callus on a medium containing cytokinins, auxin, casein hydrolysate and coconut milk, which could be induced to form multiple shoots on transfer to a medium lacking casein hydrolysate, coconut milk and auxin. Rooting of isolated shoots by treatment with an auxin mixture (indole-3-acetic acid, indole-3butyric acid and indole-3-propionie acid) and transfer of the plantlets to field have also been successful.

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