Rapid method for the preparation of an AGE-BSA standard calibrator using thermal glycation

✍ Scribed by A.D. Bhatwadekar; V.S. Ghole

• Publisher: John Wiley and Sons
• Year: 2005
• Tongue: English
• Weight: 117 KB
• Volume: 19
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/jcla.20048

✦ Synopsis

Estimation of advanced glycation end products (AGEs) by determining fluorescence is based on the use of a standard calibrator prepared by incubating bovine serum albumin (BSA) and glucose at 371C for 60 days.

In the present study we attempted to reduce the duration of incubation to 4 days by increasing the temperature to 501C. It is noteworthy that incubation at 501C resulted in the rapid production of an AGE-BSA standard calibrator within 4 days. Aminoguanidine reduced the intensity of the glycation-induced fluorescence, while the addition of lysine intensified the reaction, as shown by the calibrator incubated at 371C. The protein carbonyl content was shown to increase in the rapidly-formed standard calibrator. Thus we conclude that a simple increase in temperature and the addition of lysine (0.1M) can accelerate the process of glycation-induced fluorescence. This calibrator can be used effectively in fluorescence assays of AGEs.