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Rapid method for purification of CD56+ natural killer cells with preferential enrichment of the CD56bright+ subset

✍ Scribed by Dr. Rupert Handgretinger; Birgitta Welte; Roland Dopfer; Dietrich Niethammer

Publisher: John Wiley and Sons
Year: 1994
Tongue: English
Weight: 366 KB
Volume: 8
Category: Article
ISSN: 0887-8013
DOI: 10.1002/jcla.1860080617

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✦ Synopsis

A rapid method for the purification of CD56+ natural killer (NK) cells with the preferential enrichment of the CD56bright+ subset is described. The method is based on the adsorption of CD56' cells indirectly stained with a biotinylated antibody on an avidin-coated column.The adsorbed CD56' cells can then be squeezed out mechanically without damaging the viability or the function of the cells. Starting from peripheral mononuclear cells from adult donors, the recovery of the CD56' cells was 11.9?9.0% (n = 8), the purity 93.0&4.9% (n = lo), and the enrichment factor 7.5k1.7 (n = 8). Further phenotypic classification of the CD56' cells into CD56dim' and CD56brigh'' cells showed a preferential enrichment of the ~~56bright' phenotype with a recovery of 40.5+19.6% (n = a), a purityof 30.3&13.2%

(n = 8), and an enrichment factor of 29.8e7.2 (n = 8). In conclusion, the described method allows the rapid purification of CD56' NK cells with the preferential enrichment of the CD56brigh" subset.

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