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Rapid method for measuring physiological concentrations of 25-hydroxyvitamin D levels in blood serum

✍ Scribed by Krystyna Prószyńska; Jacek Łukaszkiewicz; Nella Jarocewicz; Roman S. Lorenc


Book ID
118319341
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
561 KB
Volume
153
Category
Article
ISSN
0009-8981

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Rapid high-performance liquid chromatogr
✍ Jacek Łukaszkiewicz; Kazimierz Kozłowski; Roman Lorenc 📂 Article 📅 1983 🏛 John Wiley and Sons 🌐 English ⚖ 281 KB

A reversed-phase HPLC method for determination of 25-hydroxycholecalciferol (25-OH D3) in serum samples is described. The method involves extraction of 25-OH D, with hexane, followed by differential resolubilization in acetonitrile. The sample is then applied directly on the CI8 bonded phase column