Rapid method for isolating detergent-insoluble outer membrane proteins from Pseudomonas aeruginosa

✍ Scribed by Dr. Madeleine Ravaoarinoro; Cristina Ciurli; Emil Toma; Richard Morisset

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 351 KB
• Volume: 15
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/elps.1150150183

✦ Synopsis

Abstract

The present study compares two techniques for isolating outer membrane proteins (OMPs) of Pseudomonas aeruginosa, Method A ‐ selective solubilization with sodium lauryl sarcosinate, and Method B ‐ isopycnic sucrose gradient centrifugation, using three criteria: the amount of proteins obtained, polypeptide patterns after sodium dodecyl sulfate‐polyacrylamide gel electrophoresis, and their practical aspects. Method A appears to be superior to Method B. It yields a higher outer membrane protein content and a similar polypeptide pattern as Method B, but is more rapid and less cumbersome.