Rapid isotyping of murine monoclonal antibodies by enzyme-linked immunofiltration assay

✍ Scribed by K. K. Hines; C. R. Clark

• Book ID: 104649611
• Publisher: Springer-Verlag
• Year: 1994
• Tongue: English
• Weight: 529 KB
• Volume: 8
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00161585

✦ Synopsis

As a part of the initial characterization of monoclonal antibodies, the isotype is routinely identified by enzyme-linked immunosorbent assay (ELISA). In the present study, we dcscribc an isotyping methodology that uses the technique referred to as enzyme-linked immunofiltration assay (ELIFA) for greatly accelerating the assay compared with ELISA. In the ELIFA method, solutions arc liltcred through a nitroccllulose mcmbranc using a controlled flow rate to bind proteins. By using this technology, the time required to isotypc a monoclonal antibody was reduced from a minimum of 4 to 8 hr using a standard ELISA assay to 30 min with ELIFA.