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Rapid Isolation of Vacuoles from Suspension-cultured Digitalis lanata Cells

✍ Scribed by Kreis, Wolfgang; Reinhard, Ernst


Book ID
121781719
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
354 KB
Volume
121
Category
Article
ISSN
0176-1617

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In order to study the accumulation and transport of rosmarinic acid in suspension cells of Coleus blumei we established an efficient method to isolate protoplasts and vacuoles. Protoplasts were disrupted by an osmotic shock in a medium with basic pH containing ethylenediamine tetraacetic acid. The r