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Rapid isolation of total acidic proteins from chromatin of various chick tissues

✍ Scribed by Elizabeth M. Wilson; Thomas C. Spelsberg


Book ID
115749555
Publisher
Elsevier Science
Year
1973
Weight
1023 KB
Volume
322
Category
Article
ISSN
0005-2795

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A rapid procedure for the isolation of total RNA from small amounts of mammalian tissue (35 to 150 mg) is described. Tissues were homogenized in the presence of RNase inhibitors but in the absence of strong detergents. Contaminants were removed by phenol/chloroform extraction and Sephadex column chr